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2013 Having Atcc Accession Number Pta, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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For all assays, n = 6 (3 male and 3 female) animals per group. A Animal survival after challenge with 1 × 10 6 pfu of intranasally-delivered A/Anhui/PA-1/2013. B Averaged clinical scores. C Percent change in body weight from date of challenge. Two statistical hypotheses were tested within each figure, with filled lines showing comparisons to the vector control and dotted lines representing tests between key experimental groups. D – F Viral load in nasal wash at Day D 1, E 3, and F 5 post-challenge. Statistical analysis performed on log-transformed data using one-way ANOVA with Dunnett’s multiple comparisons test. G H7-binding IgG antibody titers in pre-challenge ferret serum. H H7 pseudovirus neutralization capacity (IC 50 ) of pre-challenge ferret serum. G Statistical analysis performed on log-transformed data used one-way ANOVA with Dunnett’s multiple comparisons test. H Statistical analysis performed on log-transformed data using the Kruskal–Wallis test with Dunn’s multiple comparisons. Data are presented as D – G mean +/− SD or H geometric mean +/− geometric SD. IN Intranasal, IM Intramuscular, LLOD lower limit of detection, NIBSC NIBSC Influenza Antigen A/Anhui/1/2013 <t>(H7N9),</t> ns not significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Associated body temperature data in Supplementary Fig. . Source data are provided as a Source Data file.
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For all assays, n = 6 (3 male and 3 female) animals per group. A Animal survival after challenge with 1 × 10 6 pfu of intranasally-delivered A/Anhui/PA-1/2013. B Averaged clinical scores. C Percent change in body weight from date of challenge. Two statistical hypotheses were tested within each figure, with filled lines showing comparisons to the vector control and dotted lines representing tests between key experimental groups. D – F Viral load in nasal wash at Day D 1, E 3, and F 5 post-challenge. Statistical analysis performed on log-transformed data using one-way ANOVA with Dunnett’s multiple comparisons test. G H7-binding IgG antibody titers in pre-challenge ferret serum. H H7 pseudovirus neutralization capacity (IC 50 ) of pre-challenge ferret serum. G Statistical analysis performed on log-transformed data used one-way ANOVA with Dunnett’s multiple comparisons test. H Statistical analysis performed on log-transformed data using the Kruskal–Wallis test with Dunn’s multiple comparisons. Data are presented as D – G mean +/− SD or H geometric mean +/− geometric SD. IN Intranasal, IM Intramuscular, LLOD lower limit of detection, NIBSC NIBSC Influenza Antigen A/Anhui/1/2013 <t>(H7N9),</t> ns not significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Associated body temperature data in Supplementary Fig. . Source data are provided as a Source Data file.
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lactobacillus plantarum zdy 2013  (ATCC)
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For all assays, n = 6 (3 male and 3 female) animals per group. A Animal survival after challenge with 1 × 10 6 pfu of intranasally-delivered A/Anhui/PA-1/2013. B Averaged clinical scores. C Percent change in body weight from date of challenge. Two statistical hypotheses were tested within each figure, with filled lines showing comparisons to the vector control and dotted lines representing tests between key experimental groups. D – F Viral load in nasal wash at Day D 1, E 3, and F 5 post-challenge. Statistical analysis performed on log-transformed data using one-way ANOVA with Dunnett’s multiple comparisons test. G H7-binding IgG antibody titers in pre-challenge ferret serum. H H7 pseudovirus neutralization capacity (IC 50 ) of pre-challenge ferret serum. G Statistical analysis performed on log-transformed data used one-way ANOVA with Dunnett’s multiple comparisons test. H Statistical analysis performed on log-transformed data using the Kruskal–Wallis test with Dunn’s multiple comparisons. Data are presented as D – G mean +/− SD or H geometric mean +/− geometric SD. IN Intranasal, IM Intramuscular, LLOD lower limit of detection, NIBSC NIBSC Influenza Antigen A/Anhui/1/2013 <t>(H7N9),</t> ns not significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Associated body temperature data in Supplementary Fig. . Source data are provided as a Source Data file.
Lactobacillus Plantarum Zdy 2013, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ncoa3/src3 cutana™ cut&run antibody  (EpiCypher)
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For all assays, n = 6 (3 male and 3 female) animals per group. A Animal survival after challenge with 1 × 10 6 pfu of intranasally-delivered A/Anhui/PA-1/2013. B Averaged clinical scores. C Percent change in body weight from date of challenge. Two statistical hypotheses were tested within each figure, with filled lines showing comparisons to the vector control and dotted lines representing tests between key experimental groups. D – F Viral load in nasal wash at Day D 1, E 3, and F 5 post-challenge. Statistical analysis performed on log-transformed data using one-way ANOVA with Dunnett’s multiple comparisons test. G H7-binding IgG antibody titers in pre-challenge ferret serum. H H7 pseudovirus neutralization capacity (IC 50 ) of pre-challenge ferret serum. G Statistical analysis performed on log-transformed data used one-way ANOVA with Dunnett’s multiple comparisons test. H Statistical analysis performed on log-transformed data using the Kruskal–Wallis test with Dunn’s multiple comparisons. Data are presented as D – G mean +/− SD or H geometric mean +/− geometric SD. IN Intranasal, IM Intramuscular, LLOD lower limit of detection, NIBSC NIBSC Influenza Antigen A/Anhui/1/2013 <t>(H7N9),</t> ns not significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Associated body temperature data in Supplementary Fig. . Source data are provided as a Source Data file.
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va 2013  (ATCC)
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For all assays, n = 6 (3 male and 3 female) animals per group. A Animal survival after challenge with 1 × 10 6 pfu of intranasally-delivered A/Anhui/PA-1/2013. B Averaged clinical scores. C Percent change in body weight from date of challenge. Two statistical hypotheses were tested within each figure, with filled lines showing comparisons to the vector control and dotted lines representing tests between key experimental groups. D – F Viral load in nasal wash at Day D 1, E 3, and F 5 post-challenge. Statistical analysis performed on log-transformed data using one-way ANOVA with Dunnett’s multiple comparisons test. G H7-binding IgG antibody titers in pre-challenge ferret serum. H H7 pseudovirus neutralization capacity (IC 50 ) of pre-challenge ferret serum. G Statistical analysis performed on log-transformed data used one-way ANOVA with Dunnett’s multiple comparisons test. H Statistical analysis performed on log-transformed data using the Kruskal–Wallis test with Dunn’s multiple comparisons. Data are presented as D – G mean +/− SD or H geometric mean +/− geometric SD. IN Intranasal, IM Intramuscular, LLOD lower limit of detection, NIBSC NIBSC Influenza Antigen A/Anhui/1/2013 <t>(H7N9),</t> ns not significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Associated body temperature data in Supplementary Fig. . Source data are provided as a Source Data file.
Va 2013, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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For all assays, n = 6 (3 male and 3 female) animals per group. A Animal survival after challenge with 1 × 10 6 pfu of intranasally-delivered A/Anhui/PA-1/2013. B Averaged clinical scores. C Percent change in body weight from date of challenge. Two statistical hypotheses were tested within each figure, with filled lines showing comparisons to the vector control and dotted lines representing tests between key experimental groups. D – F Viral load in nasal wash at Day D 1, E 3, and F 5 post-challenge. Statistical analysis performed on log-transformed data using one-way ANOVA with Dunnett’s multiple comparisons test. G H7-binding IgG antibody titers in pre-challenge ferret serum. H H7 pseudovirus neutralization capacity (IC 50 ) of pre-challenge ferret serum. G Statistical analysis performed on log-transformed data used one-way ANOVA with Dunnett’s multiple comparisons test. H Statistical analysis performed on log-transformed data using the Kruskal–Wallis test with Dunn’s multiple comparisons. Data are presented as D – G mean +/− SD or H geometric mean +/− geometric SD. IN Intranasal, IM Intramuscular, LLOD lower limit of detection, NIBSC NIBSC Influenza Antigen A/Anhui/1/2013 (H7N9), ns not significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Associated body temperature data in Supplementary Fig. . Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Intranasal replicon vaccine establishes mucosal immunity and protects against H5N1 and H7N9 influenza

doi: 10.1038/s41467-025-64829-6

Figure Lengend Snippet: For all assays, n = 6 (3 male and 3 female) animals per group. A Animal survival after challenge with 1 × 10 6 pfu of intranasally-delivered A/Anhui/PA-1/2013. B Averaged clinical scores. C Percent change in body weight from date of challenge. Two statistical hypotheses were tested within each figure, with filled lines showing comparisons to the vector control and dotted lines representing tests between key experimental groups. D – F Viral load in nasal wash at Day D 1, E 3, and F 5 post-challenge. Statistical analysis performed on log-transformed data using one-way ANOVA with Dunnett’s multiple comparisons test. G H7-binding IgG antibody titers in pre-challenge ferret serum. H H7 pseudovirus neutralization capacity (IC 50 ) of pre-challenge ferret serum. G Statistical analysis performed on log-transformed data used one-way ANOVA with Dunnett’s multiple comparisons test. H Statistical analysis performed on log-transformed data using the Kruskal–Wallis test with Dunn’s multiple comparisons. Data are presented as D – G mean +/− SD or H geometric mean +/− geometric SD. IN Intranasal, IM Intramuscular, LLOD lower limit of detection, NIBSC NIBSC Influenza Antigen A/Anhui/1/2013 (H7N9), ns not significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Associated body temperature data in Supplementary Fig. . Source data are provided as a Source Data file.

Article Snippet: Plates were coated with either H5(H5N1)(A/Vietnam/1203/2004) obtained from Immune Technology (#IT-003-0051p) or H7N9 (A/Anhui/1/2013) HA from Sino Biological (#40103-V08H) followed by blocking for 1 h. Each serum or BAL sample was diluted 1:40 or 1:4, respectively, and then serially 1:2 to create a 14-point dilution curve for each sample.

Techniques: Plasmid Preparation, Control, Transformation Assay, Binding Assay, Neutralization